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human leukemia monocytic type 1 thp 1 cells  (ATCC)


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    ATCC human leukemia monocytic type 1 thp 1 cells
    ( a ) Immunofluorescence images of BMECs in a multiwell plate stained for nuclei (blue) and ICAM-1 (magenta) following 16–17 h treatment with 50% hECSR medium + 50% conditioned medium <t>from</t> <t>THP-1</t> macrophages. The THP-1s had been treated with or without 1×10 8 BEVs ml -1 for 24 h prior to collection of the conditioned medium. ( b ) Barplot of fluorescence intensity of ICAM-1 divided by cell count and normalized to the BMECs exposed to conditioned medium from untreated THP-1s. Performed unpaired two-tailed t-test. ( c ) Immunofluorescence images of BMEC/BPLC co-cultures immunostained for cell nuclei (blue) and claudin-5 (cyan) following 16–17 h treatment with 50% hECSR medium + 50% conditioned medium from THP-1 macrophages. Gaps in claudin-5 staining are circled with dotted lines (yellow). ( d ) Barplot of µSiM co-culture system permeability to lucifer yellow dye. Performed unpaired two-tailed t-test. ( e )–( k ) Cytokine analysis of conditioned medium from THP-1 macrophages that was collected following 24 h treatment with or without 1×10 6 BEVs ml -1 , 1×10 8 BEVs ml -1 , or 10 ng ml -1 LPS. Performed one-way ANOVA with Dunnett post-hoc test for each cytokine. ( e ) Barplot of TNF-α concentration. ( f ) Barplot of IL-1β concentration. ( g ) Barplot of IL-1RA concentration. ( h ) Barplot of IL-2 concentration. ( i ) Barplot of IL-8 concentration. ( j ) Barplot of GM-CSF concentration. ( k ) Barplot of MCP-1 concentration. Scale bars = 100 µm. Error bars = s.d. ✱ = p < 0.05, ✱✱ = p < 0.01, ✱✱✱ = p < 0.001, ✱✱✱✱ = p < 0.0001.
    Human Leukemia Monocytic Type 1 Thp 1 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 20870 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human leukemia monocytic type 1 thp 1 cells/product/ATCC
    Average 99 stars, based on 20870 article reviews
    human leukemia monocytic type 1 thp 1 cells - by Bioz Stars, 2026-02
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    1) Product Images from "Bacterial extracellular vesicles indirectly destabilize a human stem cell–derived blood–brain barrier on-chip through pro-inflammatory stimulation of immune cells"

    Article Title: Bacterial extracellular vesicles indirectly destabilize a human stem cell–derived blood–brain barrier on-chip through pro-inflammatory stimulation of immune cells

    Journal: bioRxiv

    doi: 10.64898/2026.01.23.701361

    ( a ) Immunofluorescence images of BMECs in a multiwell plate stained for nuclei (blue) and ICAM-1 (magenta) following 16–17 h treatment with 50% hECSR medium + 50% conditioned medium from THP-1 macrophages. The THP-1s had been treated with or without 1×10 8 BEVs ml -1 for 24 h prior to collection of the conditioned medium. ( b ) Barplot of fluorescence intensity of ICAM-1 divided by cell count and normalized to the BMECs exposed to conditioned medium from untreated THP-1s. Performed unpaired two-tailed t-test. ( c ) Immunofluorescence images of BMEC/BPLC co-cultures immunostained for cell nuclei (blue) and claudin-5 (cyan) following 16–17 h treatment with 50% hECSR medium + 50% conditioned medium from THP-1 macrophages. Gaps in claudin-5 staining are circled with dotted lines (yellow). ( d ) Barplot of µSiM co-culture system permeability to lucifer yellow dye. Performed unpaired two-tailed t-test. ( e )–( k ) Cytokine analysis of conditioned medium from THP-1 macrophages that was collected following 24 h treatment with or without 1×10 6 BEVs ml -1 , 1×10 8 BEVs ml -1 , or 10 ng ml -1 LPS. Performed one-way ANOVA with Dunnett post-hoc test for each cytokine. ( e ) Barplot of TNF-α concentration. ( f ) Barplot of IL-1β concentration. ( g ) Barplot of IL-1RA concentration. ( h ) Barplot of IL-2 concentration. ( i ) Barplot of IL-8 concentration. ( j ) Barplot of GM-CSF concentration. ( k ) Barplot of MCP-1 concentration. Scale bars = 100 µm. Error bars = s.d. ✱ = p < 0.05, ✱✱ = p < 0.01, ✱✱✱ = p < 0.001, ✱✱✱✱ = p < 0.0001.
    Figure Legend Snippet: ( a ) Immunofluorescence images of BMECs in a multiwell plate stained for nuclei (blue) and ICAM-1 (magenta) following 16–17 h treatment with 50% hECSR medium + 50% conditioned medium from THP-1 macrophages. The THP-1s had been treated with or without 1×10 8 BEVs ml -1 for 24 h prior to collection of the conditioned medium. ( b ) Barplot of fluorescence intensity of ICAM-1 divided by cell count and normalized to the BMECs exposed to conditioned medium from untreated THP-1s. Performed unpaired two-tailed t-test. ( c ) Immunofluorescence images of BMEC/BPLC co-cultures immunostained for cell nuclei (blue) and claudin-5 (cyan) following 16–17 h treatment with 50% hECSR medium + 50% conditioned medium from THP-1 macrophages. Gaps in claudin-5 staining are circled with dotted lines (yellow). ( d ) Barplot of µSiM co-culture system permeability to lucifer yellow dye. Performed unpaired two-tailed t-test. ( e )–( k ) Cytokine analysis of conditioned medium from THP-1 macrophages that was collected following 24 h treatment with or without 1×10 6 BEVs ml -1 , 1×10 8 BEVs ml -1 , or 10 ng ml -1 LPS. Performed one-way ANOVA with Dunnett post-hoc test for each cytokine. ( e ) Barplot of TNF-α concentration. ( f ) Barplot of IL-1β concentration. ( g ) Barplot of IL-1RA concentration. ( h ) Barplot of IL-2 concentration. ( i ) Barplot of IL-8 concentration. ( j ) Barplot of GM-CSF concentration. ( k ) Barplot of MCP-1 concentration. Scale bars = 100 µm. Error bars = s.d. ✱ = p < 0.05, ✱✱ = p < 0.01, ✱✱✱ = p < 0.001, ✱✱✱✱ = p < 0.0001.

    Techniques Used: Immunofluorescence, Staining, Fluorescence, Cell Characterization, Two Tailed Test, Co-Culture Assay, Permeability, Concentration Assay



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    ( a ) Immunofluorescence images of BMECs in a multiwell plate stained for nuclei (blue) and ICAM-1 (magenta) following 16–17 h treatment with 50% hECSR medium + 50% conditioned medium <t>from</t> <t>THP-1</t> macrophages. The THP-1s had been treated with or without 1×10 8 BEVs ml -1 for 24 h prior to collection of the conditioned medium. ( b ) Barplot of fluorescence intensity of ICAM-1 divided by cell count and normalized to the BMECs exposed to conditioned medium from untreated THP-1s. Performed unpaired two-tailed t-test. ( c ) Immunofluorescence images of BMEC/BPLC co-cultures immunostained for cell nuclei (blue) and claudin-5 (cyan) following 16–17 h treatment with 50% hECSR medium + 50% conditioned medium from THP-1 macrophages. Gaps in claudin-5 staining are circled with dotted lines (yellow). ( d ) Barplot of µSiM co-culture system permeability to lucifer yellow dye. Performed unpaired two-tailed t-test. ( e )–( k ) Cytokine analysis of conditioned medium from THP-1 macrophages that was collected following 24 h treatment with or without 1×10 6 BEVs ml -1 , 1×10 8 BEVs ml -1 , or 10 ng ml -1 LPS. Performed one-way ANOVA with Dunnett post-hoc test for each cytokine. ( e ) Barplot of TNF-α concentration. ( f ) Barplot of IL-1β concentration. ( g ) Barplot of IL-1RA concentration. ( h ) Barplot of IL-2 concentration. ( i ) Barplot of IL-8 concentration. ( j ) Barplot of GM-CSF concentration. ( k ) Barplot of MCP-1 concentration. Scale bars = 100 µm. Error bars = s.d. ✱ = p < 0.05, ✱✱ = p < 0.01, ✱✱✱ = p < 0.001, ✱✱✱✱ = p < 0.0001.
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    human leukemia monocytic type 1 thp 1 cells cells the american type culture collection  (ATCC)
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    ( a ) Immunofluorescence images of BMECs in a multiwell plate stained for nuclei (blue) and ICAM-1 (magenta) following 16–17 h treatment with 50% hECSR medium + 50% conditioned medium <t>from</t> <t>THP-1</t> macrophages. The THP-1s had been treated with or without 1×10 8 BEVs ml -1 for 24 h prior to collection of the conditioned medium. ( b ) Barplot of fluorescence intensity of ICAM-1 divided by cell count and normalized to the BMECs exposed to conditioned medium from untreated THP-1s. Performed unpaired two-tailed t-test. ( c ) Immunofluorescence images of BMEC/BPLC co-cultures immunostained for cell nuclei (blue) and claudin-5 (cyan) following 16–17 h treatment with 50% hECSR medium + 50% conditioned medium from THP-1 macrophages. Gaps in claudin-5 staining are circled with dotted lines (yellow). ( d ) Barplot of µSiM co-culture system permeability to lucifer yellow dye. Performed unpaired two-tailed t-test. ( e )–( k ) Cytokine analysis of conditioned medium from THP-1 macrophages that was collected following 24 h treatment with or without 1×10 6 BEVs ml -1 , 1×10 8 BEVs ml -1 , or 10 ng ml -1 LPS. Performed one-way ANOVA with Dunnett post-hoc test for each cytokine. ( e ) Barplot of TNF-α concentration. ( f ) Barplot of IL-1β concentration. ( g ) Barplot of IL-1RA concentration. ( h ) Barplot of IL-2 concentration. ( i ) Barplot of IL-8 concentration. ( j ) Barplot of GM-CSF concentration. ( k ) Barplot of MCP-1 concentration. Scale bars = 100 µm. Error bars = s.d. ✱ = p < 0.05, ✱✱ = p < 0.01, ✱✱✱ = p < 0.001, ✱✱✱✱ = p < 0.0001.
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    Image Search Results


    ( a ) Immunofluorescence images of BMECs in a multiwell plate stained for nuclei (blue) and ICAM-1 (magenta) following 16–17 h treatment with 50% hECSR medium + 50% conditioned medium from THP-1 macrophages. The THP-1s had been treated with or without 1×10 8 BEVs ml -1 for 24 h prior to collection of the conditioned medium. ( b ) Barplot of fluorescence intensity of ICAM-1 divided by cell count and normalized to the BMECs exposed to conditioned medium from untreated THP-1s. Performed unpaired two-tailed t-test. ( c ) Immunofluorescence images of BMEC/BPLC co-cultures immunostained for cell nuclei (blue) and claudin-5 (cyan) following 16–17 h treatment with 50% hECSR medium + 50% conditioned medium from THP-1 macrophages. Gaps in claudin-5 staining are circled with dotted lines (yellow). ( d ) Barplot of µSiM co-culture system permeability to lucifer yellow dye. Performed unpaired two-tailed t-test. ( e )–( k ) Cytokine analysis of conditioned medium from THP-1 macrophages that was collected following 24 h treatment with or without 1×10 6 BEVs ml -1 , 1×10 8 BEVs ml -1 , or 10 ng ml -1 LPS. Performed one-way ANOVA with Dunnett post-hoc test for each cytokine. ( e ) Barplot of TNF-α concentration. ( f ) Barplot of IL-1β concentration. ( g ) Barplot of IL-1RA concentration. ( h ) Barplot of IL-2 concentration. ( i ) Barplot of IL-8 concentration. ( j ) Barplot of GM-CSF concentration. ( k ) Barplot of MCP-1 concentration. Scale bars = 100 µm. Error bars = s.d. ✱ = p < 0.05, ✱✱ = p < 0.01, ✱✱✱ = p < 0.001, ✱✱✱✱ = p < 0.0001.

    Journal: bioRxiv

    Article Title: Bacterial extracellular vesicles indirectly destabilize a human stem cell–derived blood–brain barrier on-chip through pro-inflammatory stimulation of immune cells

    doi: 10.64898/2026.01.23.701361

    Figure Lengend Snippet: ( a ) Immunofluorescence images of BMECs in a multiwell plate stained for nuclei (blue) and ICAM-1 (magenta) following 16–17 h treatment with 50% hECSR medium + 50% conditioned medium from THP-1 macrophages. The THP-1s had been treated with or without 1×10 8 BEVs ml -1 for 24 h prior to collection of the conditioned medium. ( b ) Barplot of fluorescence intensity of ICAM-1 divided by cell count and normalized to the BMECs exposed to conditioned medium from untreated THP-1s. Performed unpaired two-tailed t-test. ( c ) Immunofluorescence images of BMEC/BPLC co-cultures immunostained for cell nuclei (blue) and claudin-5 (cyan) following 16–17 h treatment with 50% hECSR medium + 50% conditioned medium from THP-1 macrophages. Gaps in claudin-5 staining are circled with dotted lines (yellow). ( d ) Barplot of µSiM co-culture system permeability to lucifer yellow dye. Performed unpaired two-tailed t-test. ( e )–( k ) Cytokine analysis of conditioned medium from THP-1 macrophages that was collected following 24 h treatment with or without 1×10 6 BEVs ml -1 , 1×10 8 BEVs ml -1 , or 10 ng ml -1 LPS. Performed one-way ANOVA with Dunnett post-hoc test for each cytokine. ( e ) Barplot of TNF-α concentration. ( f ) Barplot of IL-1β concentration. ( g ) Barplot of IL-1RA concentration. ( h ) Barplot of IL-2 concentration. ( i ) Barplot of IL-8 concentration. ( j ) Barplot of GM-CSF concentration. ( k ) Barplot of MCP-1 concentration. Scale bars = 100 µm. Error bars = s.d. ✱ = p < 0.05, ✱✱ = p < 0.01, ✱✱✱ = p < 0.001, ✱✱✱✱ = p < 0.0001.

    Article Snippet: Human leukemia monocytic type 1 (THP-1) cells (ATCC) were obtained from the lab of Dr. Karin Wuertz-Kozak.

    Techniques: Immunofluorescence, Staining, Fluorescence, Cell Characterization, Two Tailed Test, Co-Culture Assay, Permeability, Concentration Assay